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PHAGOCYTOSIS OF 32 P‐LABELLED E. COLI BY HUMAN POLYMORPHONUCLEAR CELLS (PMN)
Author(s) -
MIDTVEDT TORE,
MELBY KJETIL
Publication year - 1978
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1978.tb00031.x
Subject(s) - phagocytosis , microbiology and biotechnology , yield (engineering) , polymorphonuclear leukocyte , chemistry , strain (injury) , granulocyte , human blood , in vitro , immunology , biology , biochemistry , materials science , anatomy , physiology , metallurgy
A system for the study of phagocytosis by human polymorphonuclear cells (PMN) is presented. The leucocytes are harvested from heparinized whole blood by the Bøyum method and transferred to glass tubes to yield glass adherent monolayers of leucocytes, approximately 80% of which were PMN. A strain of E. coli labelled by 32 P serves as test organism.