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ANTIGENIC CHARACTERISTICS OF MORAXELLA NONLIQUEFACIENS FIMBRIAE IN DOUBLE IMMUNODIFFUSION
Author(s) -
FRØHOLM LEIF ODDVAR
Publication year - 1978
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1978.tb00028.x
Subject(s) - fimbria , antiserum , pilus , antigen , microbiology and biotechnology , immunodiffusion , moraxella , chemistry , urea , biology , bacteria , biochemistry , escherichia coli , immunology , genetics , gene
Rabbit antisera against purified fimbriae (pili) from Moraxella nonliquefaciens detected three fimbrial antigenic components, one or two of which appeared to be present in other fimbriated strains of M. nonliquefaciens and the closely related M. bovis. Maximal precipitation with the antisera required some denaturation of the antigen. Ultrasonication, repeated freeze‐thawing, heating, and agents like KBr, NaSCN and urea were effective in liberating the antigen in diffusible forms. The morphology of the fimbriae was altered by heat treatment in 1 M KBr.