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IMMUNOCHEMICAL STUDIES ON THE SPECIFIC AGGLUTINOGENS OF STAPHYLOCOCCUS AUREUS
Author(s) -
GROV A.,
FLANDROIS J. P.,
FLEURETTE J.,
OEDING P.
Publication year - 1978
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1978.tb00023.x
Subject(s) - staphylococcus aureus , antigen , microbiology and biotechnology , antiserum , agar , chemistry , bacteria , precipitin , antibody , immunodiffusion , biology , immunology , genetics
The specific Staphylococcus aureus agglutinogen h 1 has been purified and shown to be a protein with a molecular weight of about 95,000. Chemical analysis revealed all the common amino acids, except tyrosine and the sulphur‐containing ones. The purified h 1 antigen was strongly immunogenic in rabbits. The antiserum produced one precipitation line on double diffusion in agar against a suspension of bacteria. It also agglutinated bacteria of the h 1 ‐containing strains, as well as tanned sheep erythrocytes sensitized with h 1 , in high dilutions. Antibodies to other known staphylococcal antigens were not detected. The identity between h 1 and Pillet's type 9 antigen was confirmed.