Premium
THE EFFECT OF COLOSTRUM AND COLOSTRAL ANTIBODY SIgA ON THE PHYSICO‐CHEMICAL PROPERTIES AND PHAGOCYTOSIS OF ESCHERICHIA COLI O86
Author(s) -
MAGNUSSON K. E.,
STENDAHL O.,
STJERNSTRÖM I.,
EDEBO L.
Publication year - 1978
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1978.tb00018.x
Subject(s) - colostrum , escherichia coli , phagocytosis , chemistry , bacteria , antibody , microbiology and biotechnology , biochemistry , biology , immunology , genetics , gene
A hydrophilic effect of human colostrum and colostral antibody SIgA binding on Escherichia coli 086 has been demonstrated by hydrophobic interaction chromatography on Octyl‐Sepharose and partition in a polymer two‐phase system containing dextran, poly(ethyleneglycol) and poly(ethyleneglycol)‐palmitate. Furthermore, antibody SIgA binding reduced the negative surface charge of the E. coli bacteria. The reaction between centrifuged but not further purified colostrum and bacteria yielded a complex which, compared to bacteria alone, showed decreased negative and increased positive surface charges, the latter being sensitive to pepsin. Binding of SIgA or colostrum to E. coli showed no definite effect on the attachment to and phagocytosis by polymorphonuclear cells in viiro. The effects observed are discussed in relation to the structure of SIgA.