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SYNTHETIC DISACCHARIDE‐PROTEIN ANTIGEN FOR PRODUCTION OF SPECIFIC O2 ANTISERUM FOR IMMUNOFLUORESCENCE DIAGNOSIS OF SALMONELLA
Author(s) -
SVENUNGSSON BO,
LINDBERG ALF A.
Publication year - 1978
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1978.tb00005.x
Subject(s) - antiserum , immunofluorescence , salmonella , disaccharide , antigen , indirect immunofluorescence , antibody , microbiology and biotechnology , biology , chemistry , immunology , biochemistry , bacteria , genetics
Antisera from rabbits immunized with the synthetic disaccharide paratose 1→3 mannose, representative of Salmonella O‐antigen 2. covalently linked to bovine serum albumin (BSA). were used in indirect immunofluorescence studies for the identification of Salmonella serogroup A (O‐antigen 1, 2,12) bacteria. Among 1311 enteric bacteria tested. 497 were Salmonella . The anti‐paratose 1→3 mannose‐BSA serum identified correctly all the 63 serogroup A strains tested. No positive reactions were recorded among 1248 strains representing Salmonella other than serogroup A, E, coli., Shigella, Citrobacter. Klebsiella, Enterobacter, Serratia. Proteus, Pseudomanas, Acinetobacter, Vibrio, Yersinitia and Bacteroides. The study illustrates the high specificity of the antiserum elicited by immunization with the synthetic disaccharide‐protein immunogen.