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THE EFFECT OF NALIDIXIC ACID, RIFAMPICIN AND CHLORAMPHENICOL ON THE SYNTHESIS OF PHOSPHOLIPASE C IN BACILLUS CEREUS
Author(s) -
VALLE KARL JOHAN,
PRYDZ HANS
Publication year - 1978
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1978.tb00003.x
Subject(s) - nalidixic acid , bacillus cereus , chloramphenicol , microbiology and biotechnology , rifampicin , chemistry , biology , antibiotics , bacteria , tetracycline , genetics
The effect of nalidixic acid, rifampicin and chloramphenicol on the synthesis of phospholipase C (EC 3.1.4.3) has been studied in washed Bacillus cereus cells resuspended in nutrient broth. In the absence of inhibitors, the synthesis showed a biphasic pattern. No synthesis or release of enzyme was found in the presence of chloramphenicol. When rifampicin was added, phospholipase C synthesis continued for 10–15 min. Nalidixic acid, at concentrations which inhibited DNA synthesis completely, permitted the synthesis of phospholipase C at the same rate and for a similar length of time as rifampicin.