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SCANNING ELECTRON MICROSCOPIC SURFACE MORPHOLOGY OF ISOLATED HEPATOCYTES FROM NORMAL AND PREMALIGNANT RAT LIVER
Author(s) -
Boman Dag,
Berg Trond,
Christoffersen Thoralf
Publication year - 1977
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1977.tb03879.x
Subject(s) - 2 acetylaminofluorene , collagenase , morphology (biology) , scanning electron microscope , pathology , electron microscope , hepatocyte , perfusion , cell , chemistry , biology , microbiology and biotechnology , enzyme , medicine , in vitro , biochemistry , materials science , optics , physics , composite material , genetics , microsome
Scanning electron microscopy was performed on hepatocytes isolated after collagenase perfusion of livers from rats fed 2‐acetylaminofluorene (AAF) in the diet for 6–8 weeks and on hepatocytes from control rats. The premalignant AAF‐cells showed a greater variation in cell size and shape. They also exhibited a reduced number of microvilli and more blebs and other protrusions on the surface than hepatocytes from control animals.