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A SIMPLE METHOD FOR DEMONSTRATING GEL‐PRECIPITATING HUMAN ANTIIMMUNOGLOBULIN ANTIBODIES
Author(s) -
Schalén Claes,
Christensen Poul
Publication year - 1977
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1977.tb03672.x
Subject(s) - antibody , immunodiffusion , rheumatoid arthritis , titer , radial immunodiffusion , human blood , immunology , medicine , chemistry , physiology
The use of aggregated human IgG for the detection of anti‐immunoglobulin antibodies by double immunodiffusion in gel is described. Of rheumatoid arthritis sera, 54 of 56 sera having a Waaler‐Rose test titre of ≥ 1:16 contained antibodies to aggregated IgG, in contrast to only 2 of 52 sera from blood donors. Furthermore, after 2‐mercapto‐ethanol treatment anti‐IgG was still demonstrated in 5 of 16 sera.