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EFFECT OF HUMAN LEUCOCYTE MIGRATION INHIBITORY FACTOR (LIF) ON 3‘, 5’‐CYCLIC AMP LEVELS OF PERIPHERAL BLOOD LEUCOCYTES
Author(s) -
Bendtzen K.,
Thode J.,
Madsen S. Nistrup
Publication year - 1977
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1977.tb03671.x
Subject(s) - incubation , peripheral blood mononuclear cell , peripheral blood , buffy coat , inhibitory postsynaptic potential , endocrinology , medicine , population , chemistry , peripheral , mechanism of action , in vitro , andrology , immunology , biology , biochemistry , environmental health
The possible involvement of 3′, 5′‐cyclic AMP (cyclic AMP) in the mechanism of action of leucocyte migration inhibitory factor (LIF) was tested. LIF‐treated human peripheral blood leucocytes were incubated at 37°C for various times between 0 and 22 h. The concentrations of cyclic AMP in these cultures did not differ from those in controls. Furthermore, LIF did not affect the cellular release of cyclic AMP. Neither when testing purified neutrophils, which are the prime targets of LIF action, an effect of LIF was found. Cyclic AMP levels decreased with time of incubation, whether testing mononuclear cells (92 per cent lymphocytes), purified neutrophils (98 per cent), or buffy coat cells (72 per cent neutrophils; 22 percent lymphocytes). However, a transient and as yet unexplained increase in the cyclic AMP levels of the latter mixed population of cells was manifested within 10 to 30 min of incubation.