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FREEZING OF RAT LYMPHOCYTES IV. In vitro and in vivo responses of T‐cells
Author(s) -
Hem Einar
Publication year - 1977
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1977.tb03660.x
Subject(s) - phytohaemagglutinin , mixed lymphocyte reaction , cryoprotectant , in vitro , in vivo , cryopreservation , lymph node , andrology , chemistry , microbiology and biotechnology , stimulation , immunology , biology , t cell , immune system , medicine , biochemistry , endocrinology , embryo
Rat lymph node lymphocytes have been frozen‐thawed with 10 per cent dimethyl sulphoxide as cryoprotectant. The present study shows that these cells respond well in T‐cell functional assays, such as stimulation with phytohaemagglutinin, as responder cells in the mixed lymphocyte reaction and in the local graft‐versus‐host assay. The magnitude of the responses obtained with frozen‐thawed cells was some 70–80 per cent of corresponding fresh cells.