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IMMUNO‐INDUCTIVE AND IMMUNO‐SUPPRESSIVE INFLUENCE OF HUMAN MONONUCLEAR PHAGOCYTES CULTURED IN VITRO
Author(s) -
Unsgaard G.
Publication year - 1977
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1977.tb03657.x
Subject(s) - in vitro , dna synthesis , peripheral blood mononuclear cell , antigen , monocyte , macrophage , thymidine , microbiology and biotechnology , immune system , dna , centrifugation , stimulation , mononuclear phagocyte system , biology , immunology , chemistry , biochemistry , neuroscience
DNA‐synthesis, as measured by 3 H thymidine incorporation, in immune lymphocytes from human blood stimulated in vitro with PPD was shown to require monocytes. No such requirement was demonstrated for PHA‐induced DNA‐synthesis under the same conditions. Initial packing of cells by centrifugation was beneficial for the cultures stimulated by antigen, but not for the cultures stimulated by lectin, thus indicating the necessity for contact between monocytes and lymphocytes for antigen stimulation. Monocytes and macrophages preincubated with PPD induced DNA‐synthesis in lymphocytes. Monocytes were found to be at least as capable of retaining and presenting antigen as macrophages, and autologous macrophages presented antigen more efficiently than allogeneic macrophages. DNA‐synthesis in lymphocytes, induced by adding PPD or PHA to the culture medium, was inhibited by a large number of monocytes. Macrophages caused inhibition under the same conditions, even a small number. DNA‐synthesis in lymphocytes induced by PPD‐preincubated monocytes and macrophages increased with increasing ratio of mononuclear phagocytes to lymphocytes up to a certain value. A further increase in the ratio caused inhibition, thus indicating both an immuno‐inductive and an immuno‐suppressive influence of mononuclear phagocytes.