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PRODUCTION OF MONOSPECIFIC ANTISERA AGAINST TWO VACCINIA VIRUS ANTIGENS
Author(s) -
Gurvin Ingmund,
Haukenes Gunnar
Publication year - 1977
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1977.tb03645.x
Subject(s) - antiserum , vaccinia , antigen , virology , hela , immunization , biology , virus , immunoelectrophoresis , microbiology and biotechnology , chemistry , cell culture , immunology , biochemistry , recombinant dna , genetics , gene
Vaccinia vims antigen was prepared from infected rabbit lung fibroblast monolayers grown in Eagle's medium supplemented with serum obtained from the rabbit going to be used for immunization. Monospecific rabbit antisera against the L and S antigens were produced by immunization with immunoprecipitates cut out from the agarose after quantitative line Immunoelectrophoresis using antigen produced in HeLa cells.