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PHAGOCYTOSIS OF HEAT‐KILLED RADIOLABELLED MYCOBACTERIA IN HUMAN MONONUCLEAR PHAGOCYTES CULTURED IN VITRO
Author(s) -
Viken K. E.,
Unsgaard G.,
Ødegaard A.
Publication year - 1977
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1977.tb03626.x
Subject(s) - phagocytosis , incubation , in vitro , microbiology and biotechnology , biology , peripheral blood mononuclear cell , chemotaxis , intracellular , mycobacterium , digestion (alchemy) , lymphocyte , bacteria , incubation period , immunology , chemistry , biochemistry , genetics , receptor , chromatography
Human mononuclear phagocytes cultured in vitro for 8 days were exposed to 125 I‐labelled, heat‐killed Mycobacterium triviale. The microorganisms were apparently engulfed, but no digestion occurred within a period of 16 days after the engulfment, measured as release of radioactivity to the medium and observed microscopically. Attempts were made to stimulate intracellular digestion of the bacteria. Pre‐incubation with BCG‐stimulated lymphocytes or with supernatants from BCG‐stimulated lymphocyte cultures did not increase the digestive ability of the cells. However, pre‐incubation with BCG‐stimulated lymphocytes or with supernatants caused detachment of the cells during the following digestion period, probably due to a cytotoxic effect of autologous, transformed lymphocytes on macrophages. When the macrophages were cultured in the presence of autologous lymphocytes and BCG, a similar effect was found.