Premium
ISOTOPE‐LABELLED α‐AMINO‐ISOBUTYRIC ACID AS AN INDICATOR IN CYTOTOXICITY TESTS
Author(s) -
Lyberg Torstein
Publication year - 1977
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1977.tb03613.x
Subject(s) - antiserum , isobutyric acid , trypan blue , chemistry , cytotoxicity , biochemistry , biology , in vitro , immunology , antibody
The effect of rabbit antisera on the uptake and release of α‐amino‐isobutyric acid (AIBA) in LS‐ and P‐388 cells was investigated. In the presence of homologous antiserum and complement, the uptake of AIBA was inhibited. In the absence of complement, no effect of antiserum was seen. Using prelabelled cells, the efflux of AIBA was greatly accelerated in the presence of antiserum and complement. The AIBA‐uptake method was compared with the trypan blue exclusion test and the 51 Cr release technique. The AIBA‐uptake method was more sensitive in quantitative cytotoxic studies.