Premium
RABBIT POLYMORPHONUCLEAR LEUKOCYTE MIGRATION IN VITRO IN RESPONSE TO LIPOPOLYSACCHARIDES FROM BACTEROIDES, FUSOBACTERIUM AND VEILLONELLA
Author(s) -
SVEEN KJELL.
Publication year - 1977
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1977.tb01992.x
Subject(s) - veillonella , fusobacterium , microbiology and biotechnology , bacteroides , in vitro , biology , immunology , chemistry , bacteria , streptococcus , biochemistry , genetics
Purified lipopolysaccharides (LPS) from strains of Bacteroides, Fusobacterium and Veillonella incubated with guinea pig serum, were tested for chemotactic activity against rabbit polymorphonuclear leukocytes (PMNs) in modified Boyden chambers. Comparisons were made to a Salmonella LPS ( S. enteriditis S‐795). Submicrogram amounts of LPS induced positive chemotaxis, and a typical dose‐response relationship up to certain dose levels was observed. The difference in chemotactic activity between the Veillonella LPS and LPS‐S‐795 was not statistically significant. The Fusobacterium LPS showed either a non‐significant or a highly significantly lower chemotactic capacity than LPS‐S‐795. The Bacteroides LPS were also clearly chemotactic, but considerable less when compared to the Salmonella LPS. When serum was not added, the LPS preparations showed no chemotactic activity.