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TYPE CLASSIFICATION OF GROUP B STREPTOCOCCI BY THE FLUORESCENT ANTIBODY TEST
Author(s) -
BEVANGER LARS,
MELAND JOHAN A.
Publication year - 1977
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1977.tb01990.x
Subject(s) - serotype , conjugate , microbiology and biotechnology , antibody , antigen , fluorescein isothiocyanate , strain (injury) , group a , group b , direct fluorescent antibody , biology , chemistry , virology , fluorescence , immunology , medicine , mathematical analysis , physics , mathematics , anatomy , quantum mechanics
Fluorescein‐isothiocyanate conjugates were prepared from rabbit anti‐type Ia, Ib, II and III sera, and used to classify 90 strains of group B streptococci isolated from the genital tract of puerperal women. Of the 84 strains that were typeable, the numbers belonging to serotypes Ia, Ib, Ic, II and III, were 7, 14, 6, 37 and 20, respectively. The anti‐type Ib conjugate showed cross‐reactivity with 21 of the type II strains and one of the type III strains. Absorption of the anti‐type Ib conjugate with bacteria of a type Ic strain and testing of enzymedigested streptococci indicated cross‐reactivity due to antibody to the Ibc protein antigen of group B streptococci. The potential usefulness of this antigen in the classification of type II and type III strains is discussed.