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SURFACE‐CHARGE CHARACTERISTICS OF SMOOTH AND ROUGH SALMONELLA TYPHIMURIUM BACTERIA DETERMINED BY AQUEOUS TWO‐PHASE PARTITIONING AND FREE ZONE ELECTROPHORESIS
Author(s) -
STENDAHL OLLE,
EDEBO LARS,
MAGNUSSON KARLERIC,
TAGESSON CHRISTER,
HJERTÉN STELLAN
Publication year - 1977
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1977.tb01984.x
Subject(s) - bacteria , surface charge , electrophoresis , chemistry , charge density , aqueous solution , partition coefficient , salmonella , aqueous two phase system , bacterial cell structure , lipopolysaccharide , partition (number theory) , peg ratio , phase (matter) , chromatography , analytical chemistry (journal) , biology , organic chemistry , physics , genetics , mathematics , quantum mechanics , combinatorics , finance , economics , endocrinology
Aqueous biphasic partitioning of Salmonella typhimurium S and R bacteria in a system containing 6.2 per cent (w/w) dextran 500 and 4.4 per cent (w/w) poly(ethyleneglycol) 6000 (PEG) was similar to the partition of the corresponding surface lipopolysaccharide (LPS). Further partition analysis with charged PEG showed that S bacteria and their LPS exposed very little charge, whereas R bacteria and their LPS showed a conspicuous negative charge at neutral pH. Free zone electrophoresis also indicated that the S bacteria have a much lower surface charge density than the R bacteria and accordingly a different surface structure. Thus, the physico‐chemical properties of the bacterial surface seem to be determined to a great extent by the characteristics of the cell surface LPS.