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INDUCTION OF LATENT HERPES SIMPLEX VIRUS TYPE 2 INFECTION IN HUMAN CERVICAL EPITHELIAL CELLS IN VITRO
Author(s) -
VESTERINEN ERVO,
LELNIKKI PAULI,
SAKSELA EERO
Publication year - 1977
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1977.tb01977.x
Subject(s) - herpes simplex virus , in vitro , virology , virus , biology , viral replication , explant culture , simplexvirus , microbiology and biotechnology , herpesviridae , viral disease , biochemistry
An in vitro method was used to induce non‐productive herpes simplex virus type 2 (HSV‐2) infections in human ecto‐ and endocervical epithelial cells. Adenine arabinoside (ara‐A) could prevent the replication of HSV‐2 in ecto‐ and endocervical cells and after removal of ara‐A from growth medium a rapid destruction of explants with development of infectious virus was observed. When ara‐A was not withdrawn until 4 days or later after in vitro infection the morphological, immunofluorescent and virological studies detected no infections. However, 2–3 weeks after inoculation there were in some cultures morphological as well as immunofluorescent signs of HSV infection without demonstrable infectious virus, indicating an incidental abortive nature of the infective process.