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CHEMICAL COMPOSITION, SEROLOGICAL REACTIVITY AND ENDOTOXICITY OF LIPOPOLYSACCHARIDES EXTRACTED IN DIFFERENT WAYS FROM BACTEROIDES FRAGILIS, BACTEROIDES MELANINOGENICUS AND BACTEROIDES ORALIS
Author(s) -
Hofstad T.,
Sveen K.,
Dahlén G.
Publication year - 1977
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1977.tb01972.x
Subject(s) - bacteroides fragilis , microbiology and biotechnology , chemistry , bacteroides , chromatography , biology , bacteria , antibiotics , genetics
Lipopolysaccharides (LPS) extracted from strains of Bacteroides fragilis, Bacteroides melaninogenicus and Bacteroides oralis with phenol‐water, trichloroacetic acid, EDTA or liquid phenolchloroform‐petroleum ether (PCP) and isolated by ultracentrifugation, varied considerably in their quantitative chemical composition. Negligible yields of LPS were obtained by PCP‐extraction. All preparations were more or less serologically active. All methods (except PCP) extracted the same O‐antigenic determinants from B. fragilis. Endotoxic activity, as measured by primary skin inflammations in rabbits, was low but was present in all preparations. Proteins (and/or lipoproteins) co‐precipitated with LPS in the ultracentrifuge.