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HUMAN INTERFERON AND CELL GROWTH INHIBITION
Author(s) -
DAHL HELEN
Publication year - 1977
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1977.tb01674.x
Subject(s) - agarose , growth inhibition , chemistry , acetic acid , biochemistry , dimer , inhibitory postsynaptic potential , cell growth , interferon , microbiology and biotechnology , chromatography , biology , virology , organic chemistry , neuroscience
Human leukocyte interferon (IF) inhibits the growth rate of homologous cells in culture. The growth inhibitory component, formerly separated from the antiviral component of human leukocyte IF by adsorption chromatography on albumin‐agarose, is investigated. The properties of the component are compared with the accepted characteristics of IF. The growth inhibition is caused by a small molecule, unstable to pH 2 treatment with HCl, but stable to trichloro‐acetic acid (TCA) and to proteolytic enzymes. The isolated growth inhibitor seems to be a dimer of molecular weight (mol wt.)˜2300. It is activated by heat treatment and lacks species specificity. The discrepancies in the properties of the growth inhibitor before before and after separation from IF are discussed.