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CROSSED IMMUNOELECTROPHORETIC ANALYSIS OF BORDETELLA PERTUSSIS ANTIGENS AND OF CORRESPONDING ANTIBODIES IN HUMAN SERA
Author(s) -
Hertz J. B.,
Høiby N.,
Andersen V.,
Bækgaard P.
Publication year - 1976
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1976.tb01957.x
Subject(s) - bordetella pertussis , antigen , immunoelectrophoresis , antiserum , antibody , virology , microbiology and biotechnology , biology , immunology , bacteria , genetics
Forty‐four antigens were demonstrated in sonicated preparations of Bordetella pertussis (B.p.), using crossed immunoelectrophoresis against antiserum obtained from rabbits. No qualitative differences between the four strains of the Danish pertussis vaccine were found. In preparations of B.p. culture medium, purified with respect to Lymphocytosis Promoting Factor (LPF) activity, one antigen was possibly related to LPF. In human sera, antibodies against five of the B.p. antigens were demonstrated by means of crossed immunoelectrophoresis with intermediate gel. Antibody production was demonstrable in children during the pertussis vaccination period and was most marked after the second vaccination.