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PROTEOLYTIC DEGRADATION OF STAPHYLOCOCCAL α‐TOXIN
Author(s) -
Dalen A. B.
Publication year - 1976
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1976.tb01943.x
Subject(s) - proteolysis , toxin , proteases , trypsin , chemistry , chymotrypsin , degradation (telecommunications) , extracellular , chromatography , biochemistry , mole , microbiology and biotechnology , enzyme , biology , telecommunications , computer science
Staphylococcal α‐toxin of mol.wt. 39,000 was degraded at an alkaline pH by staphylococcal extracellular proteases resulting in the formation of three relatively stable intermediates with mol.wt. 27,500, 23,500 and 12,000. The intermediate with mol.wt. 27,500 which existed in two charged forms, was isolated by column chromatography and found to be non‐haemolytic. Furthermore, it could be obtained by proteolysis of α‐toxin (mol.wt. 39,000) with chymotrypsin in low concentrations. This intermediate was further degraded by trypsin to the protein with mol.wt. 23,500 and 12,000.