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LOCALIZATION OF UREASE ACTIVITY IN UREAPLASMA UREALYTICUM CELLS
Author(s) -
Vinther O.
Publication year - 1976
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1976.tb01928.x
Subject(s) - urease , ureaplasma urealyticum , glutaraldehyde , cytoplasm , chemistry , urea , hydrolysis , biochemistry , chromatography , nuclear chemistry , microbiology and biotechnology , biology , mycoplasma
Measurements of urease activity of various cell fractions of U. urealyticum showed that this activity was confined to the soluble fraction of the cytoplasm. It was attempted to devise a method for electron microscopic detection of the sites of urease activity based on precipitation of electron dense MnO 2 at the alkaline pH created by hydrolysis of urea. The results obtained by this method supported the previous results indicating a cytoplasmatic localization of the urease activity in the cells. Helical ribosome patterns were observed when glutaraldehyde fixed cells were treated with the cytochemical test solutions.