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GROWTH OF RABBIT AORTIC SMOOTH MUSCLE CELLS IN SERUM FROM PATIENTS WITH JUVENILE DIABETES
Author(s) -
Ledet Thomas
Publication year - 1976
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1976.tb00148.x
Subject(s) - medicine , endocrinology , juvenile , diabetes mellitus , incubation , fructose , serum glucose , dialysis , stimulation , smooth muscle , biology , chemistry , biochemistry , genetics
The effect of human diabetic serum on the growth of rabbit arterial smooth muscle cell cultures was studied in the stationary phase of growth. The serum was obtained from young, male, non‐obese, juvenile diabetics and non‐diabetics. The experiments were carried out using dialysed as well as non‐dialysed serum. The concentration of cholesterol and triglycerides were equal in normal and diabetic serum. Media supplemented with diabetic serum from both short term and long term diabetics stimulated the outgrowth of the smooth muscle cells significantly (2p<0.01). A statistically significantly stimulation of growth was also observed using dialysed human diabetic serum (2p<0.05). Autoradiographic studies showed that the number of 3 H‐thymidine labelled cells and of cells in mitosis increased appreciably after incubation in diabetic human serum (2p<0.005). The present data show that human serum from juvenile diabetics contains a factor or factors which promote an excessive growth of arterial medial cells. The factor(s) is not lipids as hyperlipemia was not present nor is it glucose, aminoacids, fructose or ketones, as the growth effect remained after dialysis.