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VISUALIZATION OF THE BINDING OF IgG MYELOMA PROTEINS TO SOME GROUP A STREPTOCOCCI
Author(s) -
CHRISTENSEN POUL,
SOLTESZ L. VASILE,
OXELIUS VIVIANNE
Publication year - 1976
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1976.tb00020.x
Subject(s) - ferritin , antibody , myeloma protein , chemistry , immunoglobulin g , electron microscope , microbiology and biotechnology , biology , immunology , biochemistry , physics , optics
The interaction between streptococci and human IgG, which does not involve the antibody combining sites, was visualized in the electron microscope by the use of human IgG myeloma proteins and ferritin labelled rabbit anti‐human IgG antibodies. The streptococci were allowed to react with human IgG myeloma proteins and the human IgG was then marked by addition of ferritin labelled rabbit anti‐human IgG antibodies; sections of streptococci studied in electron microscopy showed localization of the ferritin on the surface of the cell wall of all the streptococci of type M1, M 3, M 12 and M 56 studied. Similar results were obtained if the ferritin‐labelled rabbit anti‐human IgG antibodies alone were allowed to react with the streptococci. The uptake of ferritin labelled rabbit anti‐human IgG antibodies could be inhibited by addition of normal rabbit serum.