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THE MACROPHAGE RESPONSE IN MICE AFTER PRIMARY AND SECONDARY IMMUNIZATION WITH SHEEP RED BLOOD CELLS
Author(s) -
Reikvam Åsmund
Publication year - 1976
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1976.tb00015.x
Subject(s) - acid phosphatase , macrophage , immune system , immunology , thymidine , biology , peritoneal cavity , lymphocyte , alkaline phosphatase , immunization , antigen , microbiology and biotechnology , chemistry , enzyme , in vitro , biochemistry , anatomy
A primary and a secondary immune response to sheep red blood cells (SRBC) was evoked in mice by intraperitoneal injection of the antigen. Macrophage proliferation and migration as well as induction of the lysosomal enzyme acid phosphatase in macrophages were assessed along with lymphocyte proliferation. The proliferative responses were evaluated by 3 H‐thymidine labelling (autoradiography). The secondary response elicited a faster and stronger lymphocyte proliferation than the primary response. This pattern was not seen to apply to the macrophages where the highest proliferative rate was found in connection with the primary response. The macrophages acquired an increased content of acid phosphatase during both types of responses, but the significantly highest level was reached in the secondary response. Large numbers of monocytes migrated into the peritoneal cavity during the first day after immunization. However, macrophage proliferation took place in resident as well as in immigrant cells.