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STUDIES ON THE TREPONEMA PALLIDUM IMMOBILIZING ACTIVITY IN NORMAL HUMAN SERUM
Author(s) -
HEDERSTEDT BENGT
Publication year - 1976
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1976.tb00012.x
Subject(s) - lysozyme , treponema , antibody , immune system , chemistry , antigen antibody complex , biology , immunology , biochemistry , syphilis , human immunodeficiency virus (hiv)
The influence of immobilizing antibody, complement and lysozyme, on the T. pallidum immobilization reactions of normal and immune sera was studied. Lysozyme shortened the lag periods and increased the reaction rates of the reactions of normal and immune sera. At high concentrations of added lysozyme, variations in the concentrations of immobilizing antibody and complement, within a wide range, did not further influence the kinetics of the two reactions. Preincubation with lysozyme did not influence the treponemes in the following immune serum immobiliation reaction provided the lysozyme was removed before the addition of antibody and complement. Normal serum was found to immobilize T. pallidum more rapidly than immune serum. This was seen also if the reaction mixtures were almost identical, the only difference being the immobilizing IgM antibody involved in the normal and the IgG antibody involved in the immune serum reaction.