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ELIMINATION OF INGESTED 32 P‐LABELLED E. COLI FROM RAT POLYMORPHONUCLEAR NEUTROPHILS (PMN)
Author(s) -
MIDTVEDT TORE,
BAARDSEN ARNE
Publication year - 1976
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1976.tb00005.x
Subject(s) - chemistry , granulocyte , microbiology and biotechnology , phagocytosis , escherichia coli , neutrophile , immunology , biology , biochemistry , in vitro , gene
A method to be used for in vitro assay of the elimination of ingested 32 P‐labelled E. coli from rat PMN monolayers has been evaluated. The rate of expulsion of bacterial label from phagocytes into the extracellular medium was found to range between 40 and 50 per cent of the total uptake 180 min after termination of ingestion. Serum enhanced the cellular uptake of bacteria, but did not affect the rate of elimination. Disintegration of the phagocytes was not found to be a problem.