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DETERMINATION OF HUMAN ANTIBODIES TO STAPHYLOCOCCAL α‐TOXIN (ANTISTAPHYLOLYSIN) BY A REVERSED SINGLE RADIAL IMMUNODIFFUSION METHOD
Author(s) -
Johnson Ulf,
Svantesson Biroitta
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1975.tb01664.x
Subject(s) - radial immunodiffusion , hemolysis , chromatography , immunodiffusion , chemistry , dilution , antibody , toxin , microbiology and biotechnology , biology , immunology , biochemistry , physics , thermodynamics
A method for the routine determination of human antibodies to staphylococcal α‐toxin (antistaphylolysin, ASTA), performed as a reversed single radial immunodiffusion, is presented. The sera to be tested are allowed to diffuse into a staphylolysin‐containing gel. A second gel containing rabbit erythrocytes is poured on top of the first gel and, after diffusion of free staphylolysin, the zones of inhibited hemolysis are measured. The method was compared with the conventional tube‐dilution method and the correlation coefficient (r) was found to be 0.95.