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DRUG EFFECTS ON HUMAN LEUCOCYTE MIGRATION AND MIGRATION INHIBITORY ACTIVITY FROM LYMPHOCYTES STIMULATED WITH CONCANAVALIN A
Author(s) -
Bendtzen Klaus
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1975.tb01663.x
Subject(s) - puromycin , diethylstilbestrol , concanavalin a , theophylline , inhibitory postsynaptic potential , in vitro , agarose , chemistry , cell migration , endocrinology , medicine , pharmacology , biology , hormone , biochemistry , protein biosynthesis
The in vitro effect of a number of drugs on human peripheral blood leucocyte migration and migration inhibitory factors produced by lymphocytes stimulated with concanavalin A was studied. Using the indirect leucocyte migration agarose technique, migration was not influenced by cyclophosphamide, diethylstilbestrol or puromycin. Theophylline (2 μg/ml) marginally inhibited, and methylprednisolone (2.5 μg/ml) markedly stimulated, cell migration under agarose. Production of leucocyte migration inhibitory activity (‐LMIA) was inhibited by theophylline (20 and 200 μg/ml) and abolished by methylprednisolone (2.5 μg/ml), cyclophosphamide (2.5 and 25 μg/ml), diethylstilbestrol (0.01 μg/ml) and puromycin (0.1 to 10 μg/ml). The influence of LMIA on migrating cells was inhibited by puromycin (1 and 10 μg/ml) and by high concentrations of diethylstilbestrol (0.1 μg/ml).