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MITOGENIC RESPONSES OF MOUSE THYMUS AND SPLEEN CELLS EFFECT OF IMMUNOADSORBENT PROCEDURES
Author(s) -
HøierMadsen M.,
Rubin Bent
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1975.tb01661.x
Subject(s) - spleen , phytohaemagglutinin , concanavalin a , antiserum , in vitro , lipopolysaccharide , biology , microbiology and biotechnology , antibody , immunology , biochemistry
The in vitro proliferative responses of mouse thymus‐ and spleen cells against the mitogens phytohaemagglutinin (PHA), concanavalin A (Con A) or lipopolysaccharide (LPS) were determined after either 1) anti‐θ antiserum and complement, or 2) immunoadsorbent column fractionation. The responses before and after 1) and 2) were compared with the reduction or enhancement in the number of θ positive cells, EA:RFC or EAC: RFC:s. It was found that the PHA response both of thymus‐ and spleen cells was equal to or higher after Ig:anti‐Ig column fractionation, whereas it was very sensitive to treatment with anti‐θ antiserum and complement. The LPS response both of thymus‐ and spleen cells was very significantly reduced after Ig:anti‐Ig column fractionation, but on the other hand, the LPS response of spleen cells was somewhat reduced after anti‐θ antiserum and complement treatment. The Con A response of thymus‐ and spleen cells was very sensitive to anti‐9 antiserum and complement treatment, but in addition it was very significantly reduced after passage through Ig:anti‐Ig columns, even though the number of θ positive cells increased at least twice and the PHA response was increased. Possible reasons for the latter findings including 1) PHA and Con A stimulate different subpopulations of T cells or 2) a different requirement of adherent cells for the two T cell mitogen in vitro responses will be discussed.