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COMPARISON OF METHODS FOR THE DETECTION OF IMMOBILIZING AND CYTOTOXIC SPERM ANTIBODIES
Author(s) -
Husted S.,
Ingerslev H. J.
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1975.tb01649.x
Subject(s) - cytotoxicity , sperm , cytotoxic t cell , agglutinin , antibody , andrology , immunology , serial dilution , antibody dependent cell mediated cytotoxicity , chemistry , biology , medicine , in vitro , biochemistry , pathology , alternative medicine , lectin
A sperm‐immobilization test, a sperm‐cytotoxicity test and a microtechnique for simultaneous detection of immobilizing and cytotoxic sperm antibodies have been compared. The immobilization test and the microtechnique were easy to use. In order to ensure reliable results of the cytotoxicity test, the evaluation of a serum had to be based on the mean spermotoxic index (STI) of three experiments. The cytotoxicity test was therefore found to be more time‐consuming and less suitable for large‐scale examinations. Among 30 sera from men with sperm agglu‐tinins, all sera with titres >64 yielded positive results by all three techniques, whereas minor differences were found in sera with lower agglutinin titres. A comparison of the tests showed that, except for one case, cytotoxic activity was observed only in sera which also had immobilizing activity and, using the microtechnique, this was observed without exception. A total of 103 sera without agglutinins revealed negative results by the three techniques. Titration of positive sera in the immobilization test and in the microtechnique using guinea‐pig and human serum, respectively, as the source of complement, showed identical results if the actual dilution of test serum in the test mixture was taken into account.