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COMPARISON BETWEEN THYMUS‐DEPENDENT AND THYMUS‐INDEPENDENT LYMPHOCYTES AS STIMULATOR CELLS IN ALLOGENEIC MIXED LYMPHOCYTE CULTURE
Author(s) -
Häyry Pekka,
Kontiainen Sirkka,
Nordling Stig,
Andersson Leif C.
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1975.tb01635.x
Subject(s) - phytohaemagglutinin , spleen , t lymphocyte , lymphocyte , immunology , mixed lymphocyte reaction , biology , microbiology and biotechnology , stimulation , t cell , endocrinology , immune system
Resting and activated DBA/2 T and non‐T spleen lymphocytes were compared with a view to their ability to stimulate CBA spleen T lymphocytes in one‐way mixed lymphocyte culture. Resting T and non‐T cells were obtained by fractionation of spleen lymphocytes in preparative free flow cell electrophoresis. Activated T and non‐T cells were obtained by stimulation of DBA/2 spleen cells with phytohaemagglutinin and E. coli lipopolysaccharide, respectively, and by fractionation of the activated cells (blasts) in one‐g velocity sedimentation. Both resting and activated DBA/2 T and non‐T cells induced MLC responses of the same order of magnitude, provided that the culture conditions were adjusted in such a way that both types of stimulator cells survived equally well.