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HISTOCHEMICAL DEMONSTRATION OF ACID β‐GLUCURONIDASE AND CARBOXYLIC ESTER HYDROLASE ACTIVITY IN SENSITIZED LYMPHOCYTES DURING LYSIS OF ALLOGENEIC FIBROBLASTS
Author(s) -
Poulsen Poul Brix,
Güttler Flemming
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1975.tb01634.x
Subject(s) - glucuronidase , staining , lysis , spleen , microbiology and biotechnology , hydrolase , incubation , cell , chemistry , beta glucuronidase , enzyme , biology , biochemistry , immunology , gene expression , genetics , gene
Sensitized spleen cells were incubated with 51 Cr‐labelled target fibroblasts. The interaction of lymphoid cells and target cells was stopped by fixation in formalin vapour. Target cell lysis was evaluated by the release of 51 Cr and the cultures were stained for acid β‐glucuronidase and carboxylic ester hydrolase. The sensitized lymphoid cells aggregated around cultured donor‐type, fibroblasts and showed a marked staining for β‐glucuronidase activity within a six‐hour incubation period. Spleen cells of unimmunized mice were negative or weakly positive for β‐glucuronidase. Staining for carboxylic ester hydrolase activity was observed only in spleen cells of allo‐immunized mice. The staining was less intense as compared with the staining for β‐glucuronidase. These observations add further support to the suggestion that lysosomal hydrolases might have a bearing on cell‐mediated target cell injury.