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DETECTION OF HUMAN ANTIBODIES TO TRICHINELLA SPIRALIS BY ENZYME‐LINKED IMMUNOSORBENT ASSAY, ELISA
Author(s) -
Engvall E.,
Ljungström I.
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1975.tb01631.x
Subject(s) - trichinella spiralis , antibody , alkaline phosphatase , immunofluorescence , trichinella , microbiology and biotechnology , enzyme , chemistry , trichinosis , biology , antigen , immunology , biochemistry , helminths
Enzyme‐linked immunosorbent assay, ELISA, was used to detect and quantitate antibodies to Trichinella spiralis in human sera. An extract of Trichinella spiralis larvae was adsorbed to polystyrene tubes. These were incubated with serum and bound antibodies were detected by anti‐immunoglobulin labelled with alkaline phosphatase. Class specific conjugates were used to detect specific IgM and IgA antibodies. ELISA was at least as sensitive as passive haem‐agglutination and more sensitive than indirect immunofluorescence.