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INTERACTION OF CONCANAVALIN A WITH HUMAN NEUTROPHIL GRANULOCYTE FUNCTION IN VITRO
Author(s) -
Koch Christian
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1975.tb01618.x
Subject(s) - concanavalin a , agglutination (biology) , granulocyte , intracellular , stimulation , ingestion , in vitro , glycoprotein , microbiology and biotechnology , chemistry , biochemistry , staphylococcus aureus , cell membrane , phagocytosis , bacteria , biology , cell , immunology , endocrinology , antigen , genetics
The ingestion of Staphylococcus aureus by human neutrophil granulocytes was inhibited by the addition of concanavalin A (Con A). Intracellular killing of the bacteria and reduction of nitroblue‐tetrazolium (NBT) was enhanced, however, indicating stimulation of oxydative metabolism by Con A. This stimulation may in part be due to agglutination of the cells, since NBT reduction was less prominent when agglutination was prevented. The effects of Con A were different from those of metabolic inhibitors of glycolysis, and the decreased ingestion is probably caused by specific binding of Con A to cell membrane glycoproteins leading to derangement in normal membrane function.