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ACTIVATED MOUSE MACROPHAGES: MORPHOLOGY, LYSOSOMAL BIOCHEMISTRY, AND MICROBICIDAL PROPERTIES OF IN VIVO AND IN VITRO ACTIVATED CELLS
Author(s) -
Reikvam Å.,
Grammeltvedt R.,
Høibv E. A.
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1975.tb01617.x
Subject(s) - in vivo , acid phosphatase , phagocytosis , in vitro , macrophage , biology , strain (injury) , toxoplasma gondii , microbiology and biotechnology , intracellular , enzyme , biochemistry , immunology , antibody , anatomy
Peritoneal macrophages harvested from mice with a 14 days old Toxoplasma (Beverly strain) infection were compared with normal macrophages activated by culture in a medium containing 50 per cent newborn calf serum (NBCS). Morphologically they seemed to be similar and numerous lysosomes appeared in both types. The lysosomal enzymes acid phosphatase and β‐glucuronidase were increased in both macrophage types. Per mg protein, acid phosphatase was increased 6.9 × in in vitro activated macrophages and 4.6 × in in vivo activated cells, and β‐glucuronidase 2.0 × and 2.2 X, respectively. The capacity of the two cell types to phagocytose the RH‐strain of T. gondii and a strain of L. monocytogenes was approximately identical. In contrast, in vivo activated macrophages were much more effective in restricting intracellular growth of the micro‐organisms. Mice initially infected with the Beverly strain and then challenged with the RH‐strain 2 weeks, 1 month, 3 months or 4 months later, showed almost 100 per cent survival rates, while normal mice all died after 5—9 days.