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PHAGOCYTOSIS AND MICROBICIDAL CAPACITY OF MOUSE MACROPHAGES NON‐SPECIFICALLY ACTIVATED IN VITRO
Author(s) -
Reikvam Å.,
Høiby E. A.
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1975.tb01616.x
Subject(s) - phagocytosis , listeria monocytogenes , in vitro , acid phosphatase , macrophage , toxoplasma gondii , intracellular , biology , microbiology and biotechnology , enzyme , chemistry , immunology , biochemistry , bacteria , antibody , genetics
Mouse macrophages cultured in a medium containing 50 per cent newborn calf serum (NBCS) were shown to undergo a characteristic development. Their size as well as their content of phase‐lucent and phase‐dense granules increased. The cell content of the lysosomal enzyme acid phosphatase was increased. In contrast, macrophages from the same source and cultured in a medium containing 50 per cent foetal calf serum (FCS) were not activated in this way. NBCS‐activated macrophages had a markedly enhanced capacity to phagocytose Toxoplasma gondii and Listeria monocytogenes, as compared with macrophages cultured in FCS. However, their ability to restrict the intracellular multiplication of these micro‐organisms was not markedly increased.