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APPEARANCE OF ACID PHOSPHATASE STAINING IN SENSITIZED LYMPHOID CELLS DURING THEIR LYSIS OF ALLOGENEIC FIBROBLASTS
Author(s) -
Poulsen Poul Brix,
Güttler Flemming,
Jørgensen Peer Nobert
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section c immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-1328
DOI - 10.1111/j.1699-0463.1975.tb01611.x
Subject(s) - staining , microbiology and biotechnology , acid phosphatase , spleen , lysis , biology , lymphocyte , antigen , chemistry , biochemistry , immunology , enzyme , genetics
Immune spleen cells or activated thymus cells were incubated with appropriate 51 Cr‐labelled target fibroblasts. The interaction of lymphoid cells and target cells was stopped by fixation in formalin vapour. Target cell lysis was evaluated by the release of 51 Cr and the cultures were stained for acid phosphatase activity by the simultaneous capture technique, using naph‐thol AS‐BI phosphoric acid and Fast red‐violet LB salt. The sensitized lymphoid cells aggregated around cultured donor‐type fibroblasts within a 4‐hour incubation period and staining for acid phosphatase activity appeared in the lymphocytes. The reaction was immuno‐logically specific, as staining was observed only in the immune spleen cells or activated thy‐mocytes incubated with target cells possessing the allo‐antigens to which the cell donor was sensitized. Specific adsorption on fibroblast monolayers syngeneic, but not allogeneic, to the H‐2 allo‐antigens against which the thymus cells were activated, reduced both the specific release of 51 Cr from labelled target cells and the number of thymus cells staining for acid phosphatase activity. Acid phosphatase staining and cytolytic activity were reduced in lymphocyte‐target cell cultures incubated in the presence of prednisolone or heparin. A significant correlation was found between target cell lysis and number of acid phosphatase‐positive spleen cells in relation to time of interaction and lymphocyte: target cell ratio.