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CYTOKINETIC VARIATIONS DURING AGEING AND REGENERATIVE GROWTH IN THE JB‐1 ASCITES TUMOUR STUDIED BY IMPULSE CYTOPHOTOMETRY
Author(s) -
Dombernowsky P.,
Bichel P.
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1975.tb01378.x
Subject(s) - ascites , feulgen stain , dna , andrology , pathology , biology , microbiology and biotechnology , chemistry , medicine , biochemistry
The variation in the DNA distributions in the murine JB‐1 ascites tumour was investigated by means of impulse cytophotometry (ICP) on days 2, 4, 7, 10 and 14 after i.p. inoculation of 2.5 times 10 6 tumour cells and on days 1, 2, 3 and 4 after aspiration of the main part of 10‐day plateau tumours. The study showed that a considerable fraction of cells with a DNA content of about 2 and 4 n (p<0.001) accumulated from day 2 to 10. During the same period the fraction of cells in S decreased significantly (p<0.001). One and two days after aspiration, a rapid rearrangement in the distribution pattern occurred, leading to a predominance of cells with S DNA content, while the fraction of cells around 2 n and 4 n decreased. Three and four days after aspiration the cells again accumulated around the 2 and 4 n values while the fraction of cells with S DNA content decreased. The ICP results were in agreement with our data obtained by autoradiography and Feulgen microspectrophotometry, and ICP is recommended as a rapid and statistically precise method in cytokinetic analysis.