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SUBCELLULAR DISTRIBUTION OF AMINO ACID NAPHTHYLAMIDASE ISOZYMES IN HUMAN LEUCOCYTES
Author(s) -
Lundgren E.,
Roos G.,
Tärnvik A.
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1975.tb01370.x
Subject(s) - isozyme , biochemistry , amino acid , differential centrifugation , biology , cytosol , microbiology and biotechnology , acid phosphatase , enzyme , alkaline phosphatase , electrophoresis , chemistry
Three isozymes (A, B and C) of amino acid naphthylamidase were found in human leucocytes. Isozyme B and C had close biochemical similarities and differed from isozyme A. Isozyme A was localized in the cytosol. Results obtained by combining electrophoresis with gradient centrifugation suggested that isozyme B originated in granules of polymorphonuclear leucocytes enriched in alkaline phosphatase. Isozyme C was found in granules of low density of polymorphonuclear as well as mononuclear leucocytes.