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PREFERENTIAL INCORPORATION OF 3H‐DEOXYCYTIDINE INTO CHICKEN BURSAL CELLS.
Author(s) -
Bäck Rune,
Linna T. Juhani
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1975.tb00165.x
Subject(s) - deoxycytidine , microgram , thymidine , deoxyuridine , nucleic acid , dna , microbiology and biotechnology , chemistry , isotope , biology , biochemistry , cancer , genetics , in vitro , physics , quantum mechanics , gemcitabine
3H‐deoxycytidine, 3H‐thymidine and 125I‐deoxyuridine were injected intravenously into different groups of chickens and mice, and lymphoid organs and intestine of the animals sampled one hour later. Incorporation of the isotopes into proliferating cells was evaluated after nucleic acid extraction and determination of specific activity of the isotopes in counts per minute per microgram of DNA (cpm/μg DNA). Labelled deoxycytidine was incorporated much better into bursal cells than the other isotopes, indicating that this isotope may preferentially label bursal lymphocytes, or a subpopulation of them.

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