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THE SEROLOGY OF PSEUDOMONAS AERUGINOSA ANALYSED BY MEANS OF QUANTITATIVE IMMUNOELECTROPHORETIC METHODS
Author(s) -
HØIBY NIELS
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1975.tb00122.x
Subject(s) - antigen , pseudomonas aeruginosa , antiserum , microbiology and biotechnology , serology , chemistry , immunoelectrophoresis , bacteria , chromatography , biology , antibody , immunology , genetics
The reproducibility of a polyvalent Pseudomonas aeruginosa antigen (St‐Ag) composed of a mixture of antigens from 4 O groups of this bacterium has been studied. Ten batches of St‐Ag were produced, and each of these and each of the 10 batches of antigens from the 4 strains of P. aeruginosa were compared with St‐Ag batch 1 by means of quantitative immunoelectrophoretic methods and a polyvalent antiserum (St‐Ab) raised against St‐Ag. Fifty‐three of the 55 antigens of St‐Ag were stable and could be reproduced with reasonable precision in all 10 batches, and 3 of the 4 strains of P. aeruginosa were stable in antigen composition in all batches. One of the strains (0‐5A) had lost 2 antigens in the last 5 batches, and the concentrations of 7 other antigens were simultaneously changed, reflecting a smooth‐rough dissociation. The disappearance of the 2 antigens in the latest 5 batches of 0‐5A was also reflected in similar changes in the antigen composition of the latest 5 batches of St‐Ag.