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PURINE METABOLISM IN NEISSERIA MENINGITIDIS
Author(s) -
JYSSUM SIDSEL
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1975.tb00118.x
Subject(s) - hypoxanthine , guanine , xanthine , guanosine , biochemistry , hypoxanthine guanine phosphoribosyltransferase , purine , chemistry , purine metabolism , neisseria meningitidis , guanosine monophosphate , inosine , guanosine triphosphate , phosphoribosyltransferase , nucleotide , biology , adenosine , enzyme , bacteria , mutant , genetics , gene
Whole cells of Neisseria meningitidis have been found to utilize exogenous radioactive hypoxanthine, guanine and xanthine. When hypoxanthine was the precursor, the pools of both the adenine and the guanine 5′‐ribonucleotides were labelled. Guanine and xanthine were utilized with labelling of the pool of the guanine 5′‐ribonucleotides only. Crude extracts from N. meningitidis were found to have activities corresponding to hypoxanthine phosphoribosyl‐transferase (EC 2.4.2.8.) and another phosphoribosyltransferase which seems to exhibit specificity for guanine and xanthine. Crude extracts phosphorylated guanosine 5′‐monophosphate to guanosine 5′‐triphosphate in the presence of adenosine 5′‐triphosphate (ATP) and MgCl 2 .