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THE INFLUENCE OF CHANGES IN THE ENVIRONMENT ON TWITCHING MOTILITY
Author(s) -
HENRICHSEN JØRGEN
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1975.tb00090.x
Subject(s) - motility , potassium nitrate , chemistry , incubation , agarose , sodium nitrate , acinetobacter calcoaceticus , food science , agar , potassium , chromatography , biochemistry , biology , bacteria , acinetobacter , inorganic chemistry , organic chemistry , microbiology and biotechnology , genetics , antibiotics
By examining medium composition and cultural conditions quantitatively it was possible to define conditions suitable to bring twitching motility about. Such conditions include the use of freshly poured, relatively thick and only slightly dried plates of a dilute medium in which the agar concentration is not too high. Incubation should take place in a humid atmosphere. In fact, everything points to the humidity as a factor of the utmost importance. Using strains of Acinetobacter calcoaceticus , it was found that a pH value of the medium adjusted to 9.0 enhances twitching motility. Spreading growth was not produced if agarose was applied instead of ordinary agars. Twitching motility was inhibited by a number of different chlorides, potassium nitrate, Tween 80 and sodium taurocholate. Possible interpretations of these observations are discussed.