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LOCALIZATION OF ANTIGENS IN THIN SECTIONS OF BACTERIA BY THE IMMUNO‐PEROXIDASE TECHNIQUE
Author(s) -
HAUGEN A.,
HELGELAND S.,
GROV A.
Publication year - 1975
Publication title -
acta pathologica microbiologica scandinavica section b microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0304-131X
DOI - 10.1111/j.1699-0463.1975.tb00075.x
Subject(s) - peroxidase , bacteria , antigen , biology , microbiology and biotechnology , chemistry , immunology , enzyme , biochemistry , genetics
The immunoperoxidase technique together with electron microscopy has been examined for the ultrastructural localization of staphylococcal protein A, the immunoferritin method being included for comparison. The results show that protein A is uniformly distributed in the whole cell wall. Both the direct and indirect methods, Fab‐ as well as Fc‐reactions, showed identical results. The immunoperoxidase method was superior to the immunoferritin method, especially when applied to thin sections of the bacteria, and the clear specificity demonstrated indicates a useful method for localization of cellular antigens.