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LEAD INDUCED INJURY OF IN VITRO CULTURED RAT FIBROBLASTS
Author(s) -
Brun Arne,
Brunk Ulf
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1974.tb03856.x
Subject(s) - in vitro , cytoplasm , cell injury , microbiology and biotechnology , programmed cell death , chemistry , fibroblast , cell , membrane , biochemistry , apoptosis , biology
In vitro cultured embryonal rat fibroblasts were used for the testing of a concentration of lead ions which would cause widespread cellular injury but not disseminated cell death. Of the tested dosages between 1 and 100 μg Pb (NO 3 ) 2 /ml cultivation medium, 25 μg was deemed suitable for further studies of the mechanism behind the cellular injury of lead. Electron microscopically there were greatly increased numbers of autolysosomes. Different possible reasons for this finding are discussed. One explanation may be lead induced lysosomal membrane damage leading to leakage of hydrolytic enzymes and cytoplasmic degradation and subsequent formation of autolysosomes. This notion is supported by a previously demonstrated lead‐induced reduction in lysosomal membrane stability using a histochemical method.