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OCCURRENCE OF IMMUNOGLOBULIN AND COMPLEMENT IN THE GLOMERULI OF RATS WITH LONG‐TERM ALLOXAN DIABETES
Author(s) -
Hägg Erik
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section a pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-4184
DOI - 10.1111/j.1699-0463.1974.tb03846.x
Subject(s) - mesangium , diabetes mellitus , glomerular mesangium , endocrinology , medicine , alloxan , antibody , pathology , chemistry , immunology , nephropathy
The occurrence of immunoglobulin G (IgG) and complement (β 1C ) in the kidneys of untreated alloxan diabetic rats has been investigated by immunofluorescent technique using ethanol fixed, paraffin embedded tissue. The results were compared with light microscopical glomerular changes described previously. IgG could be demonstrated in glomeruli (mainly in the mesangium) of almost all diabetic rats as early as one month after diabetes induction, i.e. before light microscopical glomerular lesions were present. The frequency and severity of IgG deposition did not change with increasing diabetes duration up to 12 months. There was a positive correlation between the amount of mesangial IgG and the severity of the diabetic state, as measured by diuresis. Slight amounts of mesangial IgG were also found in a few old nondiabetic controls. β 1C could not be demonstrated with certainty in glomeruli when using the paraffin embedding method. However, the use of freezing technique in a few diabetic rats permitted detection of β 1C in the mesangium of some cases.