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ISOELECTRIC ANALYSIS OF HAEMOLYSINS AND ENZYMES FROM STREPTOCOCCI OF GROUPS A, C AND G
Author(s) -
Smyth C. J.,
Fehrenbach F. J.
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1974.tb02384.x
Subject(s) - isoelectric focusing , hemolysin , chemistry , group a , esterase , streptococcus pyogenes , enzyme , group (periodic table) , streptolysin , pi , biochemistry , microbiology and biotechnology , chromatography , biology , bacteria , medicine , staphylococcus aureus , organic chemistry , genetics , virulence , gene , bacterial protein
Crude lyophilised or concentrated culture supernatant fluids from streptococci of Groups A and C were examined in detail by density gradient isoelectric focusing. Two streptolysin O components were consistently detected which had pI values of 6.0/6.1 (major form) and 7.5/7.6 (minor form) respectively. Similar components were observed in Group G material. Two NADases were also revealed with pI values of 8.4 and 8.9 (Group A) and 8.6 and 9.3 (Group C). Esterase activity, pI 5.8, was detected in Group C preparations, but not in Group A. Four DNases were resolved with pIs of 4.4, 5.8, 7.9 and 9.0, the latter two possessing RNase activity. Only the two acidic components were present in the Group C preparations examined. These DNases have been provisionally as C, A, D and B respectively. Streptokinase, pI 5.8 (Group A) and 5.4 (Group C), and hyaluronate lyase, pI 4.4 (Group A) and 4.3 (Group C) were also assayed for. Electrofocusing is suitable for quantitative preparation NADase from Group C streptococcal material.