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GAS CHROMATOGRAPHY OF BACTERIAL WHOLE CELL METHANOLYSATES.
Author(s) -
Jantzen Erik,
Bryn Klaus,
Bøvre Kjell
Publication year - 1974
Publication title -
acta pathologica microbiologica scandinavica section b microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0365-5563
DOI - 10.1111/j.1699-0463.1974.tb02373.x
Subject(s) - chromatography , chemistry , monosaccharide , fractionation , elution , anhydrous , methanol , depolymerization , gas chromatography , solvent , extraction (chemistry) , hexane , fatty acid , acetonitrile , organic chemistry
A procedure for determination of the fatty acid and monosaccharide composition of bacterial whole cell preparations has been developed. Specific elution profiles of total fatty acids, hydroxy fatty acids and monosaccharides from 1–10 mg dry cells are obtained. The method is based on the following five steps: 1. Selective depolymerization of washed, lyophilized whole cells by treatment with HCl in anhydrous methanol. 2. Trifluoroacetylation of released and solubilized monomeric compounds. 3. Fractionation of the trifluoroacetylated methanolysate by an acetonitrile/hexane two phase solvent system. 4. Analyses of the fractions by gas‐liquid chromatography. 5. Peak identification by retention characteristics, selective extraction, reduction, and by mass spectrometry.